With YMC-Triart Prep, a pH stable preparative grade HPLC stationary phase gets available. This hybrid material can be produced in lot sizes of up to several hundred kg. It is well suited for production scale applications, as it is available up to multi-ton scale.
YMC-Triart Prep is chemically stable up to pH 10.0. This provides more flexibility for method development and also allows for more efficient cleaning-in-place (CIP) procedures. From real life process development work YMC-Triart Prep has been shown to outperform traditional silica-based materials in terms of durability up to 4-fold. Longer column lifetimes lead to greater amounts of product being produced per kilogram of stationary phase. The results are: improved production procedures and reduced overall costs.
Shearing and crushing of silica particles during repacking of columns leads to the formation of fines which results in increased backpressures. By using mechanically stable, spherical particles the formation of fines can be reduced.
Here, the high mechanical stability of YMC-Triart Prep is demonstrated by means of repeated packing and unpacking of a DAC column. Even after more than 10 repacking cycles for the same material the pressure does not increase. The absence of fines is shown by the constant backpressure. This makes YMC-Triart Prep a valuable phase for method development and especially for process optimisation!
In a production process, repeated injections of a solution of insulin in human plasma/serum are carried out. Consequently, absorption of protein and other impurities to the surface of the packing material reduces the retention capacity of the column.
Methodology: At this point a wash step with an alkaline solution (e.g. 0.1 M NaOH) removed the impurities and restores the full capacity of the column.
Problem: Silica materials are unsuitable for alkaline wash conditions, because of their poor stability at high pH.
Solution: Hybrid silica based YMC-Triart Prep has an excellent stability at high pH. It is stable to alkaline wash conditions which enables longer column lifetime.
This in turn reduces production costs: Lower consumption of packing material and less downtime due to column repacking. An extension of column lifetime by a factor of > two has been achieved!
The efficiency of a preparative purification depends on the selectivity of the stationary phase and its ability to separate possible impurities from the main product. An increase in selectivity would not only improve the degree of separation of the peaks, but also reduce the cleaning costs by increasing the load on the column. Furthermore, the amount of sorbent and consumption of mobile phase could be reduced to increase the yield for a given capacity or to improve the product quality.
With YMC-Triart the output could be increased at the same time as reducing costs.
Considering the overall costs, the proposed costs to obtain 1 kg insulin (purity ≥ 98.5%, starting from an initial purity 96.5%) with YMC-Triart Prep C8-L and appropriate solvents are 19% lower than with Daisogel SP-120-10-C8-BIO. In terms of process economy YMC Triart Prep C8-L scores the lowest overall purification costs and lowest absolute costs of the stationary phase. The amount of sorbent costs represents only about 1.7% of the overall costs, which is also the lowest percentage of all phases used.
|YMC-Triart Prep C18-S||YMC-Triar Prep C8-S|
|Base Material||Organic/ inorganic silica|
|Particle Sizes||10, 15, 20 µm|
|Pore Size||12 nm / 120 Å||20 nm / 200 Å|
|Surface Area||360 m²/g||220 m²/g|
YMC-Triart Prep is produced using micro-reactor technology in the bead formation process. This results in spherical particles with a highly uniform particle and pore size distribution which contributes to a reduction of the backpressure and to more reproducibility in surface modification.
|YMC-Triart Prep Properties||Your Benefit|
Stable towards CIP-conditions
|Chemical and mechanical stability||Cost savings
High production throughput
|Uniform particle size||Improved packing
= improved chromatographic performance
|Narrow pore size distribution||Increased loadability|
|100% aqueous conditions||Flexibility
Separation of polar compounds
|Title||Document Type||Language||Phase Type||Download|
|Purification of polar compounds with YMC-Triart Prep C18-S||Application Note||E|
|Purification with YMC-Triart Prep C8||Brochure||E||Please login|
|YMC-Triart Prep Care and Use||Care and Use Instructions||E|
|MSDS YMC-Triart Bulk||MSDS||E|
|Bulk Packing Material for Preparative Chromatography||Overview||E|
Please contact YMC for pricing or specific solutions for your purification process.
Product Manager Bulk Media
ph. +49 (0)2064 427-280
YMC-Triart Prep C18-S
|YMC-Triart Prep C8-S|
|Pore Size||Particle Size||Product Code||Pore Size||Particle Size||Product Code|
|12 nm / 120 Å||10 µm||TAS12S11||
20 nm / 200 Å
|15 µm||TAS12S16||15 µm||TOS20S16|
|20 µm||TAS12S21||20 µm||TOS20S21|
Other particle and pore size combinations are on request.
Examples of existing customised material:
TAS08S11, YMC-Triart Prep C18-S, 8 nm / 80 Å, 10 µm
TOS12S11, YMC-Triart Prep C8-S, 12 nm / 120 Å, 10 µm
Typical pack sizes:
Laboratory scale: 100 g - 5 kg PE bottle
Industrial scale: 5 kg - 50 kg in double lined PE bags inside metal drum;
Larger pack sizes on request