BioPro IEX Bulk Media

BioPro IEX Bulk Media

IEX Stationary Phases for Preparative Chromatography

BioPro IEX Bulk Media

    Particle Technology

    Ion exchange chromatography (IEX) is widely used in the analysis and purification of bio-molecules. Using reversible charge-charge interactions offers several advantages in comparison to other chromatographic methods, e.g. high capacity and fast throughput. For these reasons, IEX is often used in the capture or intermediate purification of bio-molecules.

    BioPro is a series of ion exchange resins specifically designed for use in bio-chromatography. It is available as strong anion (BioPro QA) and cation exchanger (BioPro SP) or weak anion (BioPro DA) and cation exchanger (BioPro CM).

    The exchange media is based on a hydrophilic polymer matrix, with particle sizes of 10/20/30/75 µm (strong exchanger) or 60 µm (weak exchanger).

    BioPro offers a high dynamic binding capacity (DBC), together with low non-specific adsorption and excellent recovery. Currently, BioPro is manufactured in lot sizes up to 450 L.

     

    Separation of Proteins in Human Serum on BioPro QA and commercial Q Type Products
    Separation of a Standard Text Mixture of Proteins
    Excellent Dynamic Binding Capacity at High Linear Flow Rates

    High sample loading at high flow rates is determined by the dynamic binding capacity of an ion exchange resin. The dynamic binding capacity of BioPro is excellent even at high flow rates. When compared to similar competitor products it consistently exhibits a higher dynamic binding capacity. This results in higher sample loading in preparative processes.

    Cleaning in Place (CIP)

    BioPro is well suited for alkaline CIP procedures. The dynamic binding capacity (DBC) and the selectivity are unaffected by 20 cycles of CIP with 1.0 M NaOH. The recovery of proteins is maintained at 100%, which demonstrates the absence of nonspecific adsorption of proteins of this hydrophilic resin.

    Stability towards alkaline conditions

    Stability towards alkaline conditions is essential for efficient sanitizing procedures in a highly regulated environment.
    BioPro demonstrates excellent tolerance towards challenging alkaline conditions. Stability testing shows that 0.01 M NaOH is suitable for long term storage of BioPro. The high chemical stability of BioPro resins allows effective cleaning with alkaline solution and the longevity this material ensures efficient and economic purifications.

    Loading Study for BioPro QA – Proteins
    Specifications
     
    BioPro
    SmartSep
    Q10/Q20/Q30
    BioPro
    Q75
    BioPro
    SmartSep
    S10/S20/S30
    BioPro
    S75
    BioPro
    DA60
    BioPro
    CM60
    Ion exchange type Strong anion exchanger Strong cation exchanger Weak anion exchanger Weak cation exchanger
    Charged group -CH2N+(CH3)3 -(CH2)4SO3- -R-N(CH3)2 -R-COOH
    Matrix Hydrophilic porous polymer beads
    Particle Size [µm] 10, 20, 30 75 10, 20, 30 75 60 60
    pH range 2 - 12 3 - 12
    Ion exchange capacity
    [meq/mL resin]
    min. 0.08
    min. 0.10
    min. 0.08
    min. 0.10
    min. 0.08
    min. 0.10
    Dynamic binding capacity
    [mg/mL resin]
    min. 110
    (BSA)
    min. 160 (BSA)
    min. 110 (lysozyme)
    min. 160 (lysozyme)
    min. 90 (poly-IgG)
    min. 77 (poly-IgG)

     

    Advantages of BioPro Resins
    BioPro Features Your Benefit
    Excellent flow properties Low backpressure
    High production throughput
    Highly uniform particle and pore size distribution Easy and efficient column packing
    Improved chromatographic performance
    pH stability Stable towards CIP-conditions
    Flexibility in method development
    High dynamic binding capacity at high flow rates High loadability
    Process more raw material
    Flexible production capacities Column filling from one lot
    Economic resin Cost effective

     

    Examples of Possible Preparative Applications of BioPro Resins
    Antibody Purification Protein Purification Peptide Purification
    Trastuzumab Histones Insulin
    Bevacizumab Interferon  
    IgG, antibody variants Factor VIII, Factor IX  
    Title Document Type Language Phase Type Download
    Purification of Insulin and Lysozyme with BioPro SmartSep Application Note E
    Purification of monoclonal antibodies with BioPro SmartSep Application Note E
    BioPro IEX materials Brochure E
    BioPro Prep 75 um Care and Use Care and Use Instructions E
    BioPro Prep CM & DA Care and Use Care and Use Instructions E
    BioPro Screening Kit Care and Use Care and Use Instructions E
    BioPro SmartSep Prep 10, 30 um Care and Use Care and Use Instructions
    YMC-BioPro IEX Packing, Care and Use Instructions Care and Use Instructions E
    MSDS BioPro Prep Bulk MSDS E
    Bulk Packing Material for Preparative Chromatography Overview E
    Purification of biopharmaceutical proteins and peptides using ion-exchange bulk media designed for high-throughput purification Poster E Please login
    BioPro SmartSep Q und S in 10, 20 und 30 µm Product Information E Please login

    Order Now

    Please contact YMC for pricing or specific solutions for your purification process.

    Michael Ostendorf

    Product Manager Bulk Media

    ph. +49 (0)2064 427-280

    Product Particle Size Product Code
    BioPro SmartSep Q10 10 µm QSA0S10
    BioPro SmartSep Q20 20 µm QSA0S20
    BioPro SmartSep Q30 30 µm QSA0S30
    BioPro Q75 75 µm QAA0S75
    BioPro SmartSep S10 10 µm SSA0S10
    BioPro SmartSep S20 20 µm SSA0S20
    BioPro SmartSep S30 30 µm SSA0S30
    BioPro S75 75 µm SPA0S75
    BioPro DA 60 µm DAM99S60
    BioPro CM 60 µm CMM99S60

     

    Typical pack sizes:

    50 mL, 250 mL, 1 L, 5L, 25 L
    Larger or customised pack sizes are available on request.

     

    Please login to see the prices (if needed insert link to login page).

    Product Description Price (EUR) Stock
    BPCMM99S60-01PK BioPro CM60, S-60 µm, 1 mL column (5 per unit) -
    BPCMM99S60-05PK BioPro CM60, S-60 µm, 5 mL column (5 per unit) -
    BPDAM99S60-01PK BioPro DA60, S-60 µm, 1 mL column (5 per unit) -
    BPDAM99S60-05PK BioPro DA60, S-60 µm, 5 mL column (5 per unit) -
    BPIESKS99-01PK Ion Exchange Selection Kit (BioPro Q75/S75/DA60/CM60), 4 x 1 mL columns -
    BPIESKS99-05PK Ion Exchange Selection Kit (BioPro Q75/S75/DA60/CM60), 4 x 5 mL columns -
    BPQAA0S75-01PK BioPro Q75, 100 nm, S-75 µm, 1 mL column (5 per unit) -
    BPQAA0S75-05PK BioPro Q75, 100 nm, S-75 µm, 5 mL column (5 per unit) -
    BPQSA0S30-01PK BioPro SmartSep Q30, 100 nm, S-30 µm, 1 mL column (5 per unit) -
    BPQSA0S30-05PK BioPro SmartSep Q30, 100 nm, S-30 µm, 5 mL column (5 per unit) -
    BPSPA0S75-01PK BioPro S75, 100 nm, S-75 µm, 1 mL column (5 per unit) -
    BPSPA0S75-05PK BioPro S75, 100 nm, S-75 µm, 5 mL column (5 per unit) -
    BPSSA0S30-01PK BioPro SmartSep S30, 100 nm, S-30 µm, 1 mL column (5 per unit) -
    BPSSA0S30-05PK BioPro SmartSep S30, 100 nm, S-30 µm, 5 mL column (5 per unit) -
    • 2-3 days
    • 1-2 weeks
    • 3-4 weeks

    Related Categories

    Contact

    • Michael Ostendorf
    • Product Manager
    • Bulk Media
    • ph. +49 (0)2064 427-280