YMC-Triart Prep

YMC-Triart Prep

Robust and pH Stable Hybrid Silica Process Material

YMC-Triart Prep

    Improve Your Process

    YMC-Triart Prep is a pH stable and mechanically robust preparative grade HPLC stationary phase. This hybrid material can be produced in lot sizes of up to several hundred kg. It is well suited for production scale applications, as it is available up to multi-ton scale and suitable for repeated packing.



    YMC-Triart Prep is chemically stable up to pH 10.0. This provides more flexibility for method development and also allows for more efficient cleaning-in-place (CIP) procedures. From real life process development work YMC-Triart Prep has been shown to outperform traditional silica-based materials in terms of durability up to 4-fold. Longer column lifetimes lead to greater amounts of product being produced per kilogram of stationary phase. The results are: improved production procedures and reduced overall costs.



    Available Phases are:

    • YMC-Triart Prep C18-S
    • YMC-Triart Prep C8-S
    Homogeneous and uniform Particles

    YMC-Triart Prep is produced using micro-reactor technology in the bead formation process. This results in spherical particles with a highly uniform particle and pore size distribution which contributes to a reduction of the backpressure and to more reproducibility in surface modification.

    Mechanical Stability Test

    Shearing and crushing of silica particles during repacking of columns leads to the formation of fines which results in increased backpressures. By using mechanically stable, spherical particles the formation of fines can be reduced.

    Here, the high mechanical stability of YMC-Triart Prep is demonstrated by means of repeated packing and unpacking of a DAC column. Even after more than 10 repacking cycles for the same material the pressure does not increase. The absence of fines is shown by the constant backpressure. This makes YMC-Triart Prep a valuable phase for method development and especially for process optimisation!

    Cleaning in Place

    In a production process, repeated injections of a solution of insulin in human plasma/serum are carried out. Consequently, absorption of protein and other impurities to the surface of the packing material reduces the retention capacity of the column.

    Methodology: At this point a wash step with an alkaline solution (e.g. 0.1 M NaOH) removed the impurities and restores the full capacity of the column.

    Problem: Silica materials are unsuitable for alkaline wash conditions, because of their poor stability at high pH.

    Solution: Hybrid silica based YMC-Triart Prep has an excellent stability at high pH. It is stable to alkaline wash conditions which enables longer column lifetime.

    This in turn reduces production costs: Lower consumption of packing material and less downtime due to column repacking. An extension of column lifetime by a factor of > two has been achieved!

    Purification of Insulin with YMC-Triart Prep C8 (experimental study by major pharm. company)

    The efficiency of a preparative purification depends on the selectivity of the stationary phase and its ability to separate possible impurities from the main product. An increase in selectivity would not only improve the degree of separation of the peaks, but also reduce the cleaning costs by increasing the load on the column. Furthermore, the amount of sorbent and consumption of mobile phase could be reduced to increase the yield for a given capacity or to improve the product quality.

    With YMC-Triart the output could be increased at the same time as reducing costs.

    Considering the overall costs, the proposed costs to obtain 1 kg insulin (purity ≥ 98.5%, starting from an initial purity 96.5%) with YMC-Triart Prep C8-L and appropriate solvents are 19% lower than with Daisogel SP-120-10-C8-BIO. In terms of process economy YMC Triart Prep C8-L scores the lowest overall purification costs and lowest absolute costs of the stationary phase. The amount of sorbent costs represents only about 1.7% of the overall costs, which is also the lowest percentage of all phases used.

    Purification of Polar Compounds

    Challenging Task

    The use of 100% water as an eluent has been a challenge in HPLC separation for decades. Even today, many C18 materials suffer from unacceptable short lifetimes, as a result of the C18 chains collapsing which drastically reduces the separation performance. As a pioneer in the field of aqueous compatible phases, YMC has offered suitable products since the 1980’s for stable separations under aqueous conditions.


    The ability to work with 100% aqueous conditions opens the door to extend the purification range to include polar compounds efficiently and economically. Due to their equivalent selectivity properties, processes can be easily scaled up from analytical to preparative scale.

    In addition, desalting can be performed using a RP-C18 phase to reduce one step within the downstream processing. YMC-Triart Prep combines high mechanical strength with chemical stability to ensure robust, long-lasting and economic purifications.


    Purification of Peptides with Full Flexibility

    Purification is the most critical step in the manufacturing process of peptide therapeutics. The right choice of chromatography media is crucial for cost-effective production. With its wide pH range (pH 2-10), YMC-Triart Prep C18-S provides you with full flexibility in the method development of peptide purification. Simple scale-up procedures ensure the reproducible result at manufacture-scale. A method for the purification of liraglutide with high resolution (antidiabetic peptide therapeutic, marketed by Novo Nordisk as Victoza®.) was successfully developed with YMC-Triart Prep C18-S under alkaline condition. The purity obtained for the target compound was 99.5%.


    Title Document Type Language Phase Type Download
    Purification of Peptides with Full Flexibility Application Note E
    Purification of polar compounds with YMC-Triart Prep C18-S Application Note E
    Purification of Insulin with YMC-Triart Prep Brochure E Please login
    YMC-Triart Prep Packing Materials Brochure E
    YMC-Triart Prep C18-S/C8-S Care and Use Care and Use Instructions E
    MSDS YMC-Triart Bulk MSDS E
    Bulk Packing Materials for Preparative Chromatography Overview E

    Request Now

    Please contact YMC for pricing or specific solutions for your purification process.

    Michael Ostendorf

    Product Manager Bulk Media


    ph. +49 (0)2064 427-280



    YMC-Triart Prep C18-S

    YMC-Triart Prep C8-S
    Pore Size Particle Size Product Code Pore Size Particle Size Product Code
    12 nm / 120 Å 10 µm TAS12S11
    20 nm / 200 Å

    10 µm TOS20S11
    15 µm TAS12S16 15 µm TOS20S16
    20 µm TAS12S21 20 µm TOS20S21

    Other particle and pore size combinations are available on request.
    Examples of existing customised materials:
    TAS08S11, YMC-Triart Prep C18-S, 8 nm / 80 Å, 10 µm
    TOS12S11, YMC-Triart Prep C8-S, 12 nm / 120 Å, 10 µm


    Typical pack sizes:

    Laboratory scale: smallest amount is 100 grams up to 4 kg PE bottle
    Industrial scale: more than 4 kg in double lined PE bags inside metal drums  (10 or 25 kg drums)

    Related Categories


    • Michael Ostendorf
    • Product Manager
    • Bulk Media
    • ph. +49 (0)2064 427-280