HPLC/UHPLC columns for SEC

YMC-Pack Diol is available in four tightly controlled porosities and is, therefore, suitable for separation and molecular weight determination of a wide range of peptides, proteins, oligonucleotides, carbohydrates and other biopolymers with molecular weights of approx 200 to several hundred thousand.

YMC-SEC MAB is especially designed for the analysis of antibodies, their fragments and aggregates ro provide high resolution for the high and low molecular weight range.

Furthermore, YMC offers a wide range of column dimensions suitable for analytical to preparative scale separations.

The silica is derivatised with 1,2-dihydroxypropane and, with the variety of pore size available, allows analyses over a broad range of peptide to large proteins or antibodies.

Principles of separation

Size Exclusion Chromatography (SEC), also referred to as Gel Filtration Chromatography (GFC) or Gel Permeation Chromatography (GPC), is a mode of liquid chromatography in which the components of a mixture are separated according to differences in their size. It is an important tool for the analysis and separation of natural and synthetic biopolymers.
Molecules with shapes such as rigid rods, random chains and spheres but with the same molecular weight behave differently in SEC. The principle of separation is based on differences in the hydrodynamic radius of the molecules in solution.
Molecules with a larger radius elute earlier and those with the smallest radius are retained longer. The separation limit is such that only those compounds which differ by more than 10% in MW can be separated by SEC.

Large molecules exit the column more rapidly as they cannot permeate the porous structure of stationary phase. Smaller ones with the lowest hydrodynamic volume elute with longer retention times because they are able to penetrate some or all of the pores of the stationary phase. Molecules of intermediate size elute in an intermediate position.

Specifications

YMC-Pack Diol stationary phases are surface modified for a versatile alternative to underivatised silica. Advantages include a shorter equilibration time as well as good selectivity. Due to this short equilibration time, the Diol phase is a good choice for SEC in comparison with underivatised silica.