BioPro IEX Bulk Media
Particle Technology
Ion exchange media is widely used in the analysis and purification of biomolecules. For instance, in industrial-scale protein production processes, IEX media are often used for initial product capture and intermediate purification stages as well as the final polishing steps. Using reversible charge-charge interactions offers several advantages over in comparison to other chromatographic methods, e.g. high capacity and fast throughput.
BioPro and BioPro SmartSep form a series of ion exchange resins specifically designed for biochromatography. It is available as a strong anion exchanger (BioPro Q) and a strong cation exchanger (BioPro S).
The media is based on a hydrophilic polymer matrix, with a particle size of 10 µm, 20 µm, 30 µm and 75 µm.
BioPro offers a high dynamic binding capacity (DBC), together with low non-specific adsorption and excellent recovery. BioPro is manufactured in lot sizes up to 450 L.
Guide to IEX Media Selection
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Specifications
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BioPro
SmartSep
Q10/Q20/Q30
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BioPro
Q75
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BioPro
SmartSep
S10/S20/S30
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BioPro
S75
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| Ion exchange type | Strong anion exchanger | Strong cation exchanger | ||
| Charged group | -R-N+(CH3)3 | -R-SO3- | ||
| Matrix | Hydrophilic porous polymer beads | |||
| Particle Size [µm] | 10, 20, 30 | 75 | 10, 20, 30 | 75 |
| pH range | 2 - 12 | |||
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Ion exchange capacity
[meq/mL resin]
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min. 0.08
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min. 0.10
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min. 0.08
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min. 0.10
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Dynamic binding capacity
[mg/mL resin]
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min. 110 (BSA)
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min. 160 (BSA)
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min. 110 (lysozyme)
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min. 160 (lysozyme)
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Compression factor
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1.05-1.15
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|||
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Typical flow rate
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200-1000 cm/h, Max. 2000 cm/h | |||
/YMC-BioPro S75 x500.jpg) |
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Bio Pro S75 Particle |
BioPro S75 Particle |
Advantages of BioPro Resins
| BioPro 75 µm features | Intermediate purification and polishing | |
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Excellent dynamic binding capacity (DBC) |
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Solution for challenging separations |
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Low non-specific adsorption and high recovery |
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Increase productivity |
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High capacity with low operating pressure even at high flow rates |
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Reduced processing costs |
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Full compliances with GMP requirements |
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Short delivery time for industrial-scale quantities |
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Short delivery for industrial-scale quantities |
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Full compliance with GMP regulations |
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Reduced processing costs |
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Availability and Regulatory Support
YMC Mission Statement
YMC provides separation technology for the entire lifecycle of pharmaceutical compounds.
YMC will never knowingly change any product that is being used in a validated production process or validated analytical method.
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Worldwide availability, FDA/GMP
Regulatory support file avaible under non-disclosure agreement. Use in validated cGMP-manufacturing processes. Customised material avaible on request. DMF registerd with FDA.
Examples of Possible Preparative Applications of BioPro Resins
| Antibody Purification | Protein Purification | Peptide Purification |
| Trastuzumab | Histones | Insulin |
| Bevacizumab | Interferon | |
| IgG, antibody variants | Factor VIII, Factor IX |
Purification of monoclonal antibodies
Ion exchange (IEX) is widely used for the analysis and purification of bio-molecules such as protaeins, peptides and monoclonal antibodies.
In industrial-scale production, IEX is used for initial capture, intermediate purifications or final product pollishing.
Challenges during mAb purification
For purification of monoclonal antibodies, high demandes are required from the separating material. Factors influencing the binding characteristics of lgG are pH, linear elocity and/or salt concentration (at the time the sample is loaded onto the column.
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Purification of Monoclonal Antibodies
Examples of Preparative Applications
- Antibody Purification
- Protein Purification
- Peptide Purification
- Oligonucleotide Purification
Separation of Proteins in Human Serum on BioPro QA and commercial Q Type Products
Separation of a Standard Text Mixture of Proteins
Purification of Proteins
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Two step purification of IgY
High Dynamic Binding Capacity: The dynamic binding capacity of BioPro is excellent even at high flow rates. This results in higher sample loading in your preparative process.
Cleaning in Place: BioPro is well suited for alkaline CIP procedures.
Stability towards alkaline conditions: The high chemical stability of BioPro resins allows effective cleaning and economic purifications.
Scalability across All Particle Sizes: The consistent performance across all particle sizes is advantageous for seamless method transfer between particle sizes.
Excellent Dynamic Binding Capacity at High Linear Flow Rates
Cleaning in Place (CIP)
BioPro S75 is well suited for alkaline CIP procedures. The dynamic binding capacity (DBC) and the selectivity are unaffected by 20 cycles of CIP with 1.0 M NaOH. The recovery of proteins is maintained at 100%, which demonstrates the absence of nonspecific adsorption of proteins of this hydrophilic resin.
Stability towards alkaline conditions
Stability towards alkaline conditions is essential for efficient sanitizing procedures in a highly regulated environment.
BioPro Q75 demonstrates excellent tolerance towards challenging alkaline conditions. Stability testing shows that 0.1 M NaOH is suitable for long term storage of BioPro. The high chemical stability of BioPro resins allows effective cleaning with alkaline solution and the longevity this material ensures efficient and economic purifications.
Loading Study for BioPro QA – Proteins
Low Backpressure
| Title | Document Type | Language | Phase Type | Download |
| Purification of Insulin and Lysozyme with BioPro IEX SmartSep | Application Note | E | ||
| Purification of monoclonal antibodies with BioPro IEX SmartSep | Application Note | E | ||
| BioPro IEX materials | Brochure | E | ||
| BioPro IEX Packing, Care and Use | Care and Use Instructions | E | ||
| BioPro IEX Prep 75 um Care and Use | Care and Use Instructions | E | ||
| BioPro IEX Screening Kit Care and Use | Care and Use Instructions | E | ||
| BioPro IEX SmartSep Prep 10, 20, 30 um Care and Use | Care and Use Instructions | E | ||
| MSDS BioPro IEX Prep Bulk | MSDS | E | ||
| Bulk Packing Material for Preparative Chromatography | Overview | E | ||
| Purification of biopharmaceutical proteins and peptides using ion-exchange bulk media designed for high-throughput purification | Poster | E | Please login | |
| BioPro IEX SmartSep Q und S in 10, 20 und 30 µm | Product Information | E | Please login |
Request Now
Please contact YMC for pricing or specific solutions for your purification process.
Michael Ostendorf
Product Manager Bulk Media
ph. +49 (0)2064 427-280
| Product | Particle Size | Product Code |
| BioPro SmartSep Q10 | 10 µm | QSA0S10 |
| BioPro SmartSep Q20 | 20 µm | QSA0S20 |
| BioPro SmartSep Q30 | 30 µm | QSA0S30 |
| BioPro Q75 | 75 µm | QAA0S75 |
| BioPro SmartSep S10 | 10 µm | SSA0S10 |
| BioPro SmartSep S20 | 20 µm | SSA0S20 |
| BioPro SmartSep S30 | 30 µm | SSA0S30 |
| BioPro S75 | 75 µm | SPA0S75 |
Typical pack sizes:
50 mL, 250 mL, 1 L, 5L, 10 L, 20 L
Larger or customised pack sizes are available on request.
Regulatory support file under non-disclosure agreement. Used in validated cGMP-manufacturing processes. Customised material available on request. DMF registered with FDA.
Please login to see the prices (if needed insert link to login page).
| Product | Description | Price (EUR) | Stock |
| BPQAA0S75-01PK | BioPro IEX Q75, 100 nm, S-75 µm, 1 mL column (5 per unit) | - | |
| BPQAA0S75-05PK | BioPro IEX Q75, 100 nm, S-75 µm, 5 mL column (5 per unit) | - | |
| BPQSA0S30-01PK | BioPro IEX SmartSep Q30, 100 nm, S-30 µm, 1 mL column (5 per unit) | - | |
| BPQSA0S30-05PK | BioPro IEX SmartSep Q30, 100 nm, S-30 µm, 5 mL column (5 per unit) | - | |
| BPSPA0S75-01PK | BioPro IEX S75, 100 nm, S-75 µm, 1 mL column (5 per unit) | - | |
| BPSPA0S75-05PK | BioPro IEX S75, 100 nm, S-75 µm, 5 mL column (5 per unit) | - | |
| BPSSA0S30-01PK | BioPro IEX SmartSep S30, 100 nm, S-30 µm, 1 mL column (5 per unit) | - | |
| BPSSA0S30-05PK | BioPro IEX SmartSep S30, 100 nm, S-30 µm, 5 mL column (5 per unit) | - |
- 2-3 days
- 1-2 weeks
- 3-4 weeks
