BioPro IEX Bulk Media
Particle Technology
Ion exchange media are widely used in the analysis and purification of biomolecules. For instance, in industrial-scale protein production processes, IEX media are often used for initial product capture and intermediate purification stages as well as the final polishing steps. Using reversible charge-charge interactions offers several advantages in comparison to other chromatographic methods, e.g. high capacity and fast throughput.
BioPro IEX and BioPro IEX SmartSep form a series of ion exchange resins specifically designed for biochromatography. They are available as a strong anion exchanger (BioPro IEX Q) and a strong cation exchanger (BioPro IEX S).
The media are based on a hydrophilic polymer matrix, with a particle size of 10 µm, 20 µm, 30 µm and 75 µm.
BioPro IEX offers a high dynamic binding capacity (DBC), together with low non-specific adsorption and excellent recovery. BioPro IEX is manufactured in lot sizes up to 450 L.
Guide to IEX Media Selection
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Specifications
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BioPro IEX
SmartSep
Q10/Q20/Q30
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BioPro IEX
Q75
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BioPro IEX
SmartSep
S10/S20/S30
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BioPro IEX
S75
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| Ion exchange type | Strong anion exchanger | Strong cation exchanger | ||
| Charged group | -R-N+(CH3)3 | -R-SO3- | ||
| Matrix | Hydrophilic porous polymer beads | |||
| Particle Size [µm] | 10, 20, 30 | 75 | 10, 20, 30 | 75 |
| pH range | 2 - 12 | |||
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Ion exchange capacity
[meq/mL resin]
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min. 0.08
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min. 0.10
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min. 0.08
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min. 0.10
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Dynamic binding capacity
[mg/mL resin]
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min. 110 (BSA)
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min. 160 (BSA)
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min. 110 (lysozyme)
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min. 160 (lysozyme)
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Compression factor
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1.05-1.15
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|||
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Typical flow rate
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200-1000 cm/h, Max. 2000 cm/h | |||
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BioPro IEX S75 Particle |
BioPro IEX S75 Particle |
Advantages of BioPro IEX Resins
| BioPro IEX 75 µm features | Intermediate purification and polishing | |
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Excellent dynamic binding capacity (DBC) |
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Solution for challenging separations |
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Low non-specific adsorption and high recovery |
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Increase productivity |
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High capacity with low operating pressure even at high flow rates |
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Reduced processing costs |
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Full compliances with GMP requirements |
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Short delivery time for industrial-scale quantities |
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Short delivery for industrial-scale quantities |
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Full compliance with GMP regulations |
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Reduced processing costs |
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Availability and Regulatory Support
YMC Mission Statement
YMC provides separation technology for the entire lifecycle of pharmaceutical compounds.
YMC will never knowingly change any product that is being used in a validated production process or validated analytical method.
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Worldwide availability, FDA/GMP
Regulatory support file avaible under non-disclosure agreement. Use in validated cGMP-manufacturing processes. Customised material avaible on request. DMF registerd with FDA.
Examples of Possible Preparative Applications of BioPro Resins
| Antibody Purification | Protein Purification | Peptide Purification |
| Trastuzumab | Histones | Insulin |
| Bevacizumab | Interferon | |
| IgG, antibody variants | Factor VIII, Factor IX |
Purification of monoclonal antibodies
Ion exchange (IEX) is widely used for the analysis and purification of bio-molecules such as protaeins, peptides and monoclonal antibodies.
In industrial-scale production, IEX is used for initial capture, intermediate purifications or final product pollishing.
Challenges during mAb purification
For purification of monoclonal antibodies, high demandes are required from the separating material. Factors influencing the binding characteristics of lgG are pH, linear elocity and/or salt concentration (at the time the sample is loaded onto the column.
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Purification of Monoclonal Antibodies
Examples of Preparative Applications
- Antibody Purification
- Protein Purification
- Peptide Purification
- Oligonucleotide Purification
Separation of Proteins in Human Serum on BioPro IEX QA and commercial Q Type Products
Separation of a Standard Test Mixture of Proteins
Purification of Proteins
Two step purification of IgY
High Dynamic Binding Capacity: The dynamic binding capacity of BioPro IEX is excellent even at high flow rates. This results in higher sample loading in your preparative process.
Cleaning in Place: BioPro IEX is well suited for alkaline CIP procedures.
Stability towards alkaline conditions: The high chemical stability of BioPro IEX resins allows effective cleaning and economic purifications.
Scalability across All Particle Sizes: The consistent performance across all particle sizes is advantageous for seamless method transfer between particle sizes.
Excellent Dynamic Binding Capacity at High Linear Flow Rates
Cleaning in Place (CIP)
BioPro IEX S75 is well suited for alkaline CIP procedures. The dynamic binding capacity (DBC) and the selectivity are unaffected by 20 cycles of CIP with 1.0 M NaOH. The recovery of proteins is maintained at 100%, which demonstrates the absence of nonspecific adsorption of proteins of this hydrophilic resin.
Stability towards alkaline conditions
Cleaning-in-place (CIP) is essential for the economic use of packed chromatography columns. Efficient cleaning procedures increase the lifetime of the separation process and thereby contribute to the overall cost effectiveness. Furthermore, powerful CIP procedures strongly increase the safety and productivity of every downstream process.
IEX resins from YMC are fully compatible to typical CIP procedures. As an example, CIP studies have been performed using NaOH solutions with BioPro IEX S75/IEX Q75 as well as with BioPro IEX SmartSep S30/Q30. All YMC IEX resins maintain their performance valueseven after 100 CIP cycles.
Loading Study for BioPro QA – Proteins
Low Backpressure
| Title | Document Type | Language | Phase Type | Download |
| Purification of Insulin and Lysozyme with BioPro IEX SmartSep | Application Note | E | ||
| Purification of monoclonal antibodies with BioPro IEX SmartSep | Application Note | E | ||
| BioPro IEX materials | Brochure | E | ||
| BioPro IEX Prep 75 um Care and Use | Care and Use Instructions | E | ||
| BioPro IEX Screening Kit Care and Use | Care and Use Instructions | E | ||
| BioPro IEX SmartSep Prep 10, 20, 30 um Care and Use | Care and Use Instructions | E | ||
| MSDS BioPro IEX Prep Bulk | MSDS | E | ||
| Bulk Packing Material for Preparative Chromatography | Overview | E | ||
| Purification of biopharmaceutical proteins and peptides using ion-exchange bulk media designed for high-throughput purification | Poster | E | Please login | |
| Excellent alkaline CIP stability of YMC IEX | Product Information | E | Please login |
Request Now
Please contact YMC for pricing or specific solutions for your purification process.
Michael Ostendorf
Product Manager Bulk Media
ph. +49 (0)2064 427-280
| Product | Particle Size | Product Code |
| BioPro IEX SmartSep Q10 | 10 µm | QSA0S10 |
| BioPro IEX SmartSep Q20 | 20 µm | QSA0S20 |
| BioPro IEXSmartSep Q30 | 30 µm | QSA0S30 |
| BioPro IEX Q75 | 75 µm | QAA0S75 |
| BioPro IEX SmartSep S10 | 10 µm | SSA0S10 |
| BioPro IEX SmartSep S20 | 20 µm | SSA0S20 |
| BioPro IEX SmartSep S30 | 30 µm | SSA0S30 |
| BioPro IEX S75 | 75 µm | SPA0S75 |
Typical pack sizes:
50 mL, 250 mL, 1 L, 5L, 10 L, 20 L
Larger or customised pack sizes are available on request.
Regulatory support file under non-disclosure agreement. Used in validated cGMP-manufacturing processes. Customised material available on request. DMF registered with FDA.
Please login to see the prices (if needed insert link to login page).
| Product | Description | Price (EUR) | Stock |
| BPQAA0S75-01PK | BioPro Q75, 100 nm, S-75 µm, 1 mL column (5 per unit) | - | |
| BPQAA0S75-05PK | BioPro Q75, 100 nm, S-75 µm, 5 mL column (5 per unit) | - | |
| BPQSA0S30-01PK | BioPro IEX SmartSep Q30, 100 nm, S-30 µm, 1 mL column (5 per unit) | - | |
| BPQSA0S30-05PK | BioPro IEX SmartSep Q30, 100 nm, S-30 µm, 5 mL column (5 per unit) | - | |
| BPSPA0S75-01PK | BioPro IEX S75, 100 nm, S-75 µm, 1 mL column (5 per unit) | - | |
| BPSPA0S75-05PK | BioPro IEX S75, 100 nm, S-75 µm, 5 mL column (5 per unit) | - | |
| BPSSA0S30-01PK | BioPro IEX SmartSep S30, 100 nm, S-30 µm, 1 mL column (5 per unit) | - | |
| BPSSA0S30-05PK | BioPro IEX SmartSep S30, 100 nm, S-30 µm, 5 mL column (5 per unit) | - |
- 2-3 days
- 1-2 weeks
- 3-4 weeks
