BioPro IEX
HPLC columns for high resolution IEX applications
Ion exchange chromatography (IEX) is widely used for analysis and purification of biomolecules. BioPro ion exchange columns are specifically designed for separation of proteins, peptides and nucleic acids.
BioPro IEX columns are based on porous and non-porous hydrophilic polymer beads with low nonspecific adsorption. They also show higher binding capacity and higher recovery of biomolecules compared to conventional IEX columns. The completely spherical, monodisperse beads, together with optimal packing technology, provide high theoretical plate numbers and symmetrical peak shapes!
Available Phases are:
Specifications
|
(formerly)
|
BioPro IEX QA
(YMC-BioPro QA)
|
BioPro IEX SP
(YMC-BioPro SP)
|
BioPro IEX QF
(YMC-BioPro QA-F)
|
BioPro IEX SF
(YMC-BioPro SP-F)
|
||
| Matrix | Hydrophilic polymer (polymethacrylate) | |||||
| Particle Size | 5 µm | 3, 5 µm | ||||
| Pore Size | 100 nm / 1000 Å | Non-porous | ||||
| Charged group | -CH2N+(CH3)3 | -(CH2)3SO3- | -CH2N+(CH3)3 | -(CH2)3SO3- | ||
| Counter ion | Cl- | Na+ | Cl- | Na+ | ||
| Ion-exchange capacity | 0.075-0.100 meq/ml-resin | 0.070-0.095 meq/ml-resin | 0.075-0.110 meq/ml-resin | 0.230-0.290 meq/ml-resin | ||
| Dynamic binding capacity | 110-150 mg BSA/ml-resin | 70-100 mg human IgG/ml-resin | 12-20 mg BSA/ml-resin | 10-18 mg human IgG/ml-resin | ||
| pH range | 2.0 - 12.0 | |||||
BioPro IEX QA/SP
|
(formerly)
|
BioPro IEX QA
(YMC-BioPro QA)
|
BioPro IEX SP
(YMC-BioPro SP)
|
||
| Matrix | Hydrophilic polymer (polymethacrylate) | |||
| Particle Size | 5 µm | |||
| Pore Size | 100 nm / 1000 Å | |||
| Charged group | -CH2N+(CH3)3 | -(CH2)3SO3- | ||
| Counter ion | Cl- | Na+ | ||
| Ion-exchange capacity | 0.075-0.100 meq/ml-resin | 0.070-0.095 meq/ml-resin | ||
| Dynamic binding capacity | 110-150 mg BSA/ml-resin | 70-100 mg human IgG/ml-resin | ||
| pH range | 2.0 - 12.0 | |||
The porous type of BioPro IEX shows great absorption capacity and excellent recovery, and is especially suitable for semipreparative separations of proteins and antibodies.
Compared with conventional porous polymer anion-exchange columns, BioPro IEX phases give higher dynamic binding capacities (DBC) and recovery rates. This indicates that BioPro IEX has a much lower nonspecific adsorption compared to conventional columns.
BioPro IEX QF/SF
|
(formerly)
|
BioPro IEX QF
(YMC-BioPro QA-F)
|
BioPro IEX SF
(YMC-BioPro SP-F)
|
| Matrix | Hydrophilic polymer (polymethacrylate) | |
| Particle Size | 3, 5 µm | |
| Pore Size | Non-porous | |
| Charged group | -CH2N+(CH3)3 | -(CH2)3SO3- |
| Counter ion | Cl- | Na+ |
| Ion-exchange capacity | 0.075-0.110 meq/ml-resin | 0.230-0.290 meq/ml-resin |
| Dynamic binding capacity | 12-20 mg BSA/ml-resin | 10-18 mg human IgG/ml-resin |
| pH range | 2.0 - 12.0 | |
The non-porous BioPro IEX particles offer high efficiency, exceptional resolution, and low operating pressures.
| Title | Document Type | Language | Phase Type | Download |
| YMC Phases for Biochromatography | Brochure | E | ||
| BioPro IEX Columns Care and Use | Care and Use Instructions | E | ||
| Analytical stationary phases for BioLC from YMC | Overview | E | ||
| An Evaluation of YMC’s Strong Cation-Exchange Stationary Phase for the Analysis of Charged Isoforms of a Commercially Available Antibody Drug | Poster | E | Please login | |
| Use of Small Particle YMC SEC and IEX Materials for Improved Characterization of Monoclonal Antibodies | Poster | E | Please login |
Please login to see the prices (if needed insert link to login page).
| Product | Description | Price (EUR) | Stock |
| QAA0S05-0346WP | BioPro IEX QA, (4.6 mm i.d.), 100 nm, 5 µm, 30 x 4.6 mm | - | |
| QAA0S05-0546WP | BioPro IEX QA, (4.6 mm i.d.), 100 nm, 5 µm, 50 x 4.6 mm | - | |
| QAA0S05-1046WP | BioPro IEX QA, (4.6 mm i.d.), 100 nm, 5 µm, 100 x 4.6 mm | - | |
| QF00S03-0346WP | BioPro IEX QF, non-porous*, (4.6 mm i.d.), 3 µm, 30 x 4.6 mm | - | |
| QF00S03-0546WP | BioPro IEX QF, non-porous*, (4.6 mm i.d.), 3 µm, 50 x 4.6 mm | - | |
| QF00S03-1046WP | BioPro IEX QF, non-porous*, (4.6 mm i.d.), 3 µm, 100 x 4.6 mm | - | |
| QF00S05-0346WP | BioPro IEX QF, non-porous*, (4.6 mm i.d.), 5 µm, 30 x 4.6 mm | - | |
| QF00S05-0546WP | BioPro IEX QF, non-porous*, (4.6 mm i.d.), 5 µm, 50 x 4.6 mm | - | |
| QF00S05-1046WP | BioPro IEX QF, non-porous*, (4.6 mm i.d.), 5 µm, 100 x 4.6 mm | - | |
| QF00S05-1546WP | BioPro IEX QF, non-porous, S-5 µm, 150 x 4.6 mm | - | |
| SF00S03-0346WP | BioPro IEX SF, non-porous, S-3 µm, 30 x 4.6 mm | - | |
| SF00S03-0546WP | BioPro IEX SF, non-porous, S-3 µm, 50 x 4.6 mm | - | |
| SF00S03-1046WP | BioPro IEX SF, non-porous, S-3 µm, 100 x 4.6 mm | - | |
| SF00S05-0346WP | BioPro IEX SF, non-porous, S-5 µm, 30 x 4.6 mm | - | |
| SF00S05-0546WP | BioPro IEX SF, non-porous, S-5 µm, 50 x 4.6 mm | - | |
| SF00S05-1046WP | BioPro IEX SF, non-porous, S-5 µm, 100 x 4.6 mm | - | |
| SF00S05-1546WP | BioPro IEX SF, non-porous, S-5 µm, 150 x 4.6 mm | - | |
| SPA0S05-0346WP | BioPro IEX SP, (4.6 mm i.d.), 100 nm, 5 µm, 30 x 4.6 mm | - | |
| SPA0S05-0546WP | BioPro IEX SP, (4.6 mm i.d.), 100 nm, 5 µm, 50 x 4.6 mm | - | |
| SPA0S05-1046WP | BioPro IEX SP, (4.6 mm i.d.), 100 nm, 5 µm, 100 x 4.6 mm | - |
- 2-3 days
- 1-2 weeks
- 3-4 weeks
