BioPro IEX
HPLC columns for high resolution IEX applications
Ion exchange chromatography (IEX) is widely used for analysis and purification of biomolecules. BioPro ion exchange columns are specifically designed for separation of proteins, peptides and nucleic acids.
BioPro IEX columns are based on porous and non-porous hydrophilic polymer beads with low nonspecific adsorption. They also show higher binding capacity and higher recovery of biomolecules compared to conventional IEX columns. The completely spherical, monodisperse beads, together with optimal packing technology, provide high theoretical plate numbers and symmetrical peak shapes!
Available Phases are:
| Application | Columns | Compound name | |
| DNA fragments | BioPro IEX QF | DNA fragments | |
| Lambda DNA Hind Ⅲ/EcoRⅠ restriction fragments | BioPro IEX QF | Lambda DNA Hind III/EcoR I digest (130-23, 130 bp) | |
| Monoclonal antibody (MAb) | BioPro IEX SF | Humanized monoclonal IgG1 | |
| Peptide mapping | BioPro IEX QA | Peptide mapping | |
| Plasmid pBR322 and pBR322 Hae III restriction fragments | BioPro IEX QF | Plasmid pBR322 Hae III digest (8-587 bp) Plasmid pBR322 (4361 bp) | |
| Proteins in human serum | BioPro IEX QA | IgG Transferrin Albumin | |
| Proteins | BioPro IEX QA | Ovalbumin Trypsin inhibitor (soybean) | |
| Synthetic oligonucleotides (Single-strand DNA) | BioPro IEX QF | 5’-TCATCACACTGAATACCAAT-3’ (20 mer) 5’-GTCATCACACTGAATACCAAT-3’ (21 mer) | |
| Synthetic oligonucleotides (Single-strand RNA) | BioPro IEX QF | 5’-U(M)C(M)A(M)U(M)C(M)A(M)C(M)A(M)C(M)U(M)G(M)A(M)A(M)U(M)A(M)C(M)C(M)A(M)A(M)U(M)-3’ (20 mer) 5’-G(M)U(M)C(M)A(M)U(M)C(M)A(M)C(M)A(M)C(M)U(M)G(M)A(M)A(M)U(M)A(M)C(M)C(M)A(M)A(M)U(M)-3’ (21 mer) |
Specifications
|
(formerly)
|
BioPro IEX QA
(YMC-BioPro QA)
|
BioPro IEX SP
(YMC-BioPro SP)
|
BioPro IEX QF
(YMC-BioPro QA-F)
|
BioPro IEX SF
(YMC-BioPro SP-F)
|
||
| Matrix | Hydrophilic polymer (polymethacrylate) | |||||
| Particle Size | 5 µm | 3, 5 µm | ||||
| Pore Size | 100 nm / 1000 Å | Non-porous | ||||
| Charged group | -CH2N+(CH3)3 | -(CH2)3SO3- | -CH2N+(CH3)3 | -(CH2)3SO3- | ||
| Counter ion | Cl- | Na+ | Cl- | Na+ | ||
| Ion-exchange capacity | 0.075-0.100 meq/ml-resin | 0.070-0.095 meq/ml-resin | 0.075-0.110 meq/ml-resin | 0.230-0.290 meq/ml-resin | ||
| Dynamic binding capacity | 110-150 mg BSA/ml-resin | 70-100 mg human IgG/ml-resin | 12-20 mg BSA/ml-resin | 10-18 mg human IgG/ml-resin | ||
| pH range | 2.0 - 12.0 | |||||
BioPro IEX QA/SP
|
(formerly)
|
BioPro IEX QA
(YMC-BioPro QA)
|
BioPro IEX SP
(YMC-BioPro SP)
|
||
| Matrix | Hydrophilic polymer (polymethacrylate) | |||
| Particle Size | 5 µm | |||
| Pore Size | 100 nm / 1000 Å | |||
| Charged group | -CH2N+(CH3)3 | -(CH2)3SO3- | ||
| Counter ion | Cl- | Na+ | ||
| Ion-exchange capacity | 0.075-0.100 meq/ml-resin | 0.070-0.095 meq/ml-resin | ||
| Dynamic binding capacity | 110-150 mg BSA/ml-resin | 70-100 mg human IgG/ml-resin | ||
| pH range | 2.0 - 12.0 | |||
The porous type of BioPro IEX shows great absorption capacity and excellent recovery, and is especially suitable for semipreparative separations of proteins and antibodies.
Compared with conventional porous polymer anion-exchange columns, BioPro IEX phases give higher dynamic binding capacities (DBC) and recovery rates. This indicates that BioPro IEX has a much lower nonspecific adsorption compared to conventional columns.
BioPro IEX QF/SF
|
(formerly)
|
BioPro IEX QF
(YMC-BioPro QA-F)
|
BioPro IEX SF
(YMC-BioPro SP-F)
|
| Matrix | Hydrophilic polymer (polymethacrylate) | |
| Particle Size | 3, 5 µm | |
| Pore Size | Non-porous | |
| Charged group | -CH2N+(CH3)3 | -(CH2)3SO3- |
| Counter ion | Cl- | Na+ |
| Ion-exchange capacity | 0.075-0.110 meq/ml-resin | 0.230-0.290 meq/ml-resin |
| Dynamic binding capacity | 12-20 mg BSA/ml-resin | 10-18 mg human IgG/ml-resin |
| pH range | 2.0 - 12.0 | |
The non-porous BioPro IEX particles offer high efficiency, exceptional resolution, and low operating pressures.
| Title | Document Type | Language | Phase Type | Download |
| HPLC Analysis of Oligonucleotides with BioPro IEX QF | Application Note | E | ||
| Optimisation of oligonucleotide separations using YMC´s non porous anion exchanger BioPro IEX QF | Application Note | E | ||
| BioPro IEX Columns Care and Use | Care and Use Instructions | E | ||
| YMC Biochromatography Columns | Catalogue | E | ||
| YMC Biochromatography Columns (HQ) | Catalogue | E | ||
| Analytical stationary phases for BioLC from YMC | Overview | E | ||
| An Evaluation of YMC’s Strong Cation-Exchange Stationary Phase for the Analysis of Charged Isoforms of a Commercially Available Antibody Drug | Poster | E | Please login | |
| Optimisation of oligonucleotide separations on ion exchange chromatography | Poster | E | Please login | |
| The Principles of BioLC & YMC BioLC Phase Overview | Poster | E | ||
| Use of Small Particle YMC SEC and IEX Materials for Improved Characterization of Monoclonal Antibodies | Poster | E | Please login | |
| Characterisation of charge heterogeneity of therapeutic monoclonal antibodies via IEX-MS using BioPro IEX SF from YMC | Product Information | E |
Please login to see the prices (if needed insert link to login page).
| Product | Description | Price (EUR) | Stock |
| QAA0S05-0346WP | BioPro IEX QA, (4.6 mm ID), 100 nm, 5 µm, 30 x 4.6 mm | - | |
| QAA0S05-0546WP | BioPro IEX QA, (4.6 mm ID), 100 nm, 5 µm, 50 x 4.6 mm | - | |
| QAA0S05-1046WP | BioPro IEX QA, (4.6 mm ID), 100 nm, 5 µm, 100 x 4.6 mm | - | |
| QAA0S06-1020WT | BioPro IEX QA, semi-preparative HPLC Column (20.0 mm ID), S-06 µm, 100 x 20.0 mm | - | |
| QF00S03-0346WP | BioPro IEX QF, non-porous*, (4.6 mm ID), 3 µm, 30 x 4.6 mm | - | |
| QF00S03-0546WP | BioPro IEX QF, non-porous*, (4.6 mm ID), 3 µm, 50 x 4.6 mm | - | |
| QF00S03-1046WP | BioPro IEX QF, non-porous*, (4.6 mm ID), 3 µm, 100 x 4.6 mm | - | |
| QF00S05-0346WP | BioPro IEX QF, non-porous*, (4.6 mm ID), 5 µm, 30 x 4.6 mm | - | |
| QF00S05-0546WP | BioPro IEX QF, non-porous*, (4.6 mm ID), 5 µm, 50 x 4.6 mm | - | |
| QF00S05-1046WP | BioPro IEX QF, non-porous*, (4.6 mm ID), 5 µm, 100 x 4.6 mm | - | |
| QF00S05-1546WP | BioPro IEX QF, non-porous, S-5 µm, 150 x 4.6 mm | - | |
| QF00S06-1020WT | BioPro IEX SF, non-porous, semi-preparative HPLC Column (20.0 mm ID), S-6 µm, 100 x 20.0 mm | - | |
| QF00S06-2546WP | BioPro IEX QF, non-porous, S-6 µm, 250 x 4.6 mm | - | |
| SF00S03-0346WP | BioPro IEX SF, non-porous, S-3 µm, 30 x 4.6 mm | - | |
| SF00S03-0546WP | BioPro IEX SF, non-porous, S-3 µm, 50 x 4.6 mm | - | |
| SF00S03-1046WP | BioPro IEX SF, non-porous, S-3 µm, 100 x 4.6 mm | - | |
| SF00S03-1546WP | BioPro IEX SF, non-porous, S-3 µm, 150 x 4.6 mm | - | |
| SF00S05-0346WP | BioPro IEX SF, non-porous, S-5 µm, 30 x 4.6 mm | - | |
| SF00S05-0546WP | BioPro IEX SF, non-porous, S-5 µm, 50 x 4.6 mm | - | |
| SF00S05-1046WP | BioPro IEX SF, non-porous, S-5 µm, 100 x 4.6 mm | - | |
| SF00S05-1546WP | BioPro IEX SF, non-porous, S-5 µm, 150 x 4.6 mm | - | |
| SF00S06-1010WT | BioPro IEX SF, non-porous, semi-preparative HPLC Column (10.0 mm ID), S-6 µm, 100 x 10.0 mm | - | |
| SF00S06-1020WT | BioPro IEX SF, non-porous, semi-preparative HPLC Column (20.0 mm ID), S-6 µm, 100 x 20.0 mm | - | |
| SF00S06-1030WT | BioPro IEX SF, non-porous, semi-preparative HPLC Column (30.0 mm ID), S-6 µm, 100 x 30.0 mm | - | |
| SF00S06-2546WP | BioPro IEX SF, non-porous, S-6 µm, 250 x 4.6 mm | - | |
| SPA0S05-0346WP | BioPro IEX SP, (4.6 mm ID), 100 nm, 5 µm, 30 x 4.6 mm | - | |
| SPA0S05-0546WP | BioPro IEX SP, (4.6 mm ID), 100 nm, 5 µm, 50 x 4.6 mm | - | |
| SPA0S05-1046WP | BioPro IEX SP, (4.6 mm ID), 100 nm, 5 µm, 100 x 4.6 mm | - | |
| SPA0S06-1020WT | BioPro IEX SP, semi-preparative HPLC Column (20.0 mm ID), S-06 µm, 100 x 20.0 mm | - | |
| XRPRCP02 | Precolumn Filter 2 µm for 1/16" o.d. + Holder, PEEK (350 bar) | - | |
| XRPRCP25 | Replacement Precolumn Filter 2 µm for 1/16" o.d., PEEK (350 bar) | - |
- 2-3 days
- 1-2 weeks
- 3-4 weeks
