Semi-preparative chromatography is the link between analytical HPLC and preparative LC. Even though the chromatographic systems used for semi-preparative LC do not reach the size of preparative LC systems, the objectives remain the same:
- Purification and isolation of maximum sample quantity
- Savings in time and costs.
YMC preparative columns, packed with YMC Bulk Materials, are available in a wide variety of column sizes to accommodate virtually any preparative separation. YMC use a high-pressure slurry technique to pack all preparative columns in optional 1,000 (69 bar) or 2,000 psi (138 bar) pressure rated hardware. Standard column dimensions range from 50 to 200 mm inner diameter and 250 to 1000 mm in length. Other column sizes will be available on request.
Traditionally, irregular shaped silica, typically with particles sizes of 40-63 μm, has been successfully used for sample clean-up and fast workup after chemical synthesis. Also, this type of silica represents cost-effective media, yet with ever improved reproducibility, for standard applications.
Applying spherical silica will greatly enhance resolution - in less time, for more challenging demands. Spherical silica generates substantially less back pressure so that increased flow rates can be applied.