Hydrophobic Interaction Columns

Hydrophobic Interaction Columns

BioPro HIC

    HPLC columns for HIC

    BioPro HIC HT as well as BioPro HIC BF are based on a non-porous hydrophobic polymer. They are designed and optimised for analysis and lab-scale purification of antibodies, antibody-drug conjugates and other proteins.

    Optimised surface modification and column packing method realise high separation performance. High-throughput analysis with a faster flow rate can be achieved together with a high resolution using BioPro HIC HT. The rigid particle provides higher pressure tolerance compared to other HIC columns, which allows higher flow rates and therefore shorter analysis times.



    BioPro HIC columns are characterised by:

    • high resolutions even at high flow rates
    • short run times and high throughput
    • excellent lot-to-lot reproducibility
    • long term stability
    • no carryover effects
    Application Columns Compound name
    Drug-to-Antibody Ratio (DAR) analysis of ADC BioPro HIC BF Cysteine-conjugated ADC mimic
    Protein Analysis by HIC BioPro HIC BF Myoglobin Ribonuclease A Lysozyme α-Chymotrypsinogen A


    BioPro HIC HT
    BioPro HIC BF
    Base particle Hydrophilic polymer (polymethacrylate)
    Particle size 2.3 µm 4 µm
    Pore size Non-porous
    Functional group Butyl
    pH range 2.0 - 12.0
    Pressure limit 40 MPa / 400 bar 20 MPa / 200 bar
    Temperature range 10 - 60°C


    Title Document Type Language Phase Type Download
    HIC Analysis of Different Monoclonal Antibodies using Isopropanol as Modifier Application Note E
    HPLC Analysis of Drug-to-Antibody Ratio with BioPro HIC BF by YMC Application Note E
    High throughput DAR determination of brentuximab vedotin using YMC's BioPro HIC HT Application Note E
    BioPro HIC HT and BioPro HIC BF Care and Use Care and Use Instructions E
    YMC Biochromatography Columns Catalogue E
    YMC Biochromatography Columns (HQ) Catalogue E
    BioPro HIC HT by YMC for High Throughput Flyer E
    Analytical Stationary Phases for BioLC from YMC Overview E
    The Principles of BioLC & YMC BioLC Phase Overview Poster E
    YMC Alternatives to Existing BioLC Columns Product Information E
    YMC's HIC Phase Especially Designed for Antibodies, ADCs and Proteins Product Information E Please login
    Welcher LC-Modus ist besser für verschiedene ADC-Typen geeignet? RP oder HIC? Technical Note D
    Which LC-mode is more suitable for different ADC-types? RP or HIC? Technical Note E
    Grundlagen der Chromatographie für die Analyse und Aufreinigung von Biologika Whitepaper D Please login

    Please login to see the prices (if needed insert link to login page).

    Product Description Price (EUR) Stock
    BHB00S04-1046WT BioPro HIC BF, non-porous, HPLC column (4.6 mm ID), S-4 µm, 100 x 4.6 mm -
    BHH00SQ3-1046PTH BioPro HIC HT, non-porous, HPLC column (4.6 mm ID), S-2.3 µm, 100 x 4.6 mm -
    XRPRCP02 Precolumn Filter 2 µm for 1/16" o.d. + Holder, PEEK (350 bar) -
    XRPRCP25 Replacement Precolumn Filter 2 µm for 1/16" o.d., PEEK (350 bar) -
    XRPRCS03 Precolumn Filter 0.5 µm for 1/16" o.d. + Holder, stainless steel (600 bar) -
    XRPRCS35 Replacement Precolumn Filter 0.5 µm for 1/16" o.d., stainless steel (600 bar) -
    • 2-3 days
    • 1-2 weeks
    • 3-4 weeks

    Related Categories


    • Dr. Daniel Eßer
    • Product Manager
    • Analytical Chromatography
    • ph. +49 (0)2064 427-280