Hydrophobic Interaction Columns

Hydrophobic Interaction Columns


    HPLC columns for HIC

    BioPro HIC BF is based on a non-porous hydrophobic polymer. It is designed and optimised for analysis and lab-scale purification of antibodies, antibody-drug conjugates and other proteins.

    Optimised surface modification and column packing method realise high separation performance. High-throughput analysis with a faster flow rate can be achieved by the rigid particle and moderate backpressure.



    BioPro HIC columns are characterised by:

    • high separation performance
    • high-throughput analysis
    • excellent lot-to-lot reproducibility
    • long term stability
    • virtually no carryover effects
    Application Columns Compound name
    Drug-to-Antibody Ratio (DAR) analysis of ADC BioPro HIC BF Cysteine-conjugated ADC mimic
    Protein Analysis by HIC BioPro HIC BF Myoglobin Ribonuclease A Lysozyme α-Chymotrypsinogen A


    BioPro HIC BF
    Base particle Hydrophilic polymer (polymethacrylate)
    Particle size 4 µm
    Pore size Non-porous
    Functional group Butyl
    pH range 2.0 - 12.0
    Temperature range 10 - 60°C


    Title Document Type Language Phase Type Download
    HIC Analysis of Different Monoclonal Antibodies using Isopropanol as Modifier Application Note E
    HPLC Analysis of Drug-to-Antibody with BioPro HIC BF by YMC Application Note E
    BioPro HIC BF Care and Use Care and Use Instructions E
    YMC Biochromatography Columns Catalogue E
    YMC Biochromatography Columns (HQ) Catalogue E
    BioPro HIC BF by YMC Flyer E
    Analytical stationary phases for BioLC from YMC Overview E
    The Principles of BioLC & YMC BioLC Phase Overview Poster E
    YMC's HIC Phase Especially Designed for Antibodies, ADCs and Proteins Product Information E Please login

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    Product Description Price (EUR) Stock
    BHB00S04-1046WT BioPro HIC BF, non-porous, S-4 µm, 100 x 4.6 mm -
    XRPRCP02 Precolumn Filter 2 µm for 1/16" o.d. + Holder, PEEK (350 bar) -
    XRPRCP25 Replacement Precolumn Filter 2 µm for 1/16" o.d., PEEK (350 bar) -
    • 2-3 days
    • 1-2 weeks
    • 3-4 weeks

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    • Dr. Daniel Eßer
    • Product Manager
    • Analytical Chromatography
    • ph. +49 (0)2064 427-280