BioPro HIC
HPLC columns for HIC
BioPro HIC HT as well as BioPro HIC BF are based on a non-porous hydrophobic polymer. They are designed and optimised for analysis and lab-scale purification of antibodies, antibody-drug conjugates and other proteins.
Optimised surface modification and column packing method realise high separation performance. High-throughput analysis with a faster flow rate can be achieved together with a high resolution using BioPro HIC HT. The rigid particle provides higher pressure tolerance compared to other HIC columns, which allows higher flow rates and therefore shorter analysis times.
BioPro HIC columns are characterised by:
- high resolutions even at high flow rates
- short run times and high throughput
- excellent lot-to-lot reproducibility
- long term stability
- no carryover effects
Specifications
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BioPro HIC HT
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BioPro HIC BF
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| Base particle | Hydrophilic polymer (polymethacrylate) | |
| Particle size | 2.3 µm | 4 µm |
| Pore size | Non-porous | |
| Functional group | Butyl | |
| pH range | 2.0 - 12.0 | |
| Pressure limit | 40 MPa / 400 bar | 20 MPa / 200 bar |
| Temperature range | 10 - 60°C | |
| Title | Document Type | Language | Phase Type | Download |
| Analysis of oxidised monoclonal antibodies using BioPro HIC BF | Application Note | E | ||
| HIC Analysis of Different Monoclonal Antibodies using Isopropanol as Modifier | Application Note | E | ||
| HPLC Analysis of Drug-to-Antibody Ratio with BioPro HIC BF by YMC | Application Note | E | ||
| High throughput DAR determination of brentuximab vedotin using YMC's BioPro HIC HT | Application Note | E | ||
| Online native HIC-MS analysis of MAbs and their molecular variants using BioPro HIC BF | Application Note | E | ||
| Online native HIC-MS analysis of cys-linked ADCs using BioPro HIC BF | Application Note | E | ||
| The right HIC column for each separation- BioPro HIC HT and BioPro HIC BF | Application Note | E | ||
| BioPro HIC HT and BioPro HIC BF Care and Use | Care and Use Instructions | E | ||
| YMC Biochromatography Columns | Catalogue | E | ||
| YMC Biochromatography Columns (HQ) | Catalogue | E | ||
| BioPro HIC HT by YMC for High Throughput | Flyer | E | ||
| Analytical Stationary Phases for BioLC from YMC | Overview | E | ||
| The Principles of BioLC & YMC BioLC Phase Overview | Poster | E | ||
| Characterisation of MAbs and related products via HIC-MS | Product Information | E | ||
| Charakterisierung von monoklonalen Antikörpern und verwandten Produkten via HIC-MS | Product Information | D | ||
| YMC Alternatives to Existing BioLC Columns | Product Information | E | ||
| YMC's HIC Phase Especially Designed for Antibodies, ADCs and Proteins | Product Information | E | Please login | |
| About the resolving power of non-porous HIC columns for intact protein analysis | Technical Note | E | ||
| Welcher LC-Modus ist besser für verschiedene ADC-Typen geeignet? RP oder HIC? | Technical Note | D | ||
| Which LC-mode is more suitable for different ADC-types? RP or HIC? | Technical Note | E | ||
| Grundlagen der Chromatographie für die Analyse und Aufreinigung von Biologika | Whitepaper | D | Please login |
Online Seminar
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| Product | Description | Price (EUR) | Stock |
| BHB00S04-1046WT | BioPro HIC BF, non-porous, HPLC column (4.6 mm ID), S-4 µm, 100 x 4.6 mm | - | |
| BHH00SQ3-1046PTH | BioPro HIC HT, non-porous, HPLC column (4.6 mm ID), S-2.3 µm, 100 x 4.6 mm | - | |
| BHH00SQ3-H346PTH | BioPro HIC HT, non-porous, HPLC column (4.6 mm ID), S-2.3 µm, 33 x 4.6 mm | - | |
| XRPRCP02 | Precolumn Filter 2 µm for 1/16" OD + Holder, PEEK (350 bar) | - | |
| XRPRCP25 | Replacement Precolumn Filter 2 µm for 1/16" OD, PEEK (350 bar) | - | |
| XRPRCS03 | Precolumn Filter 0.5 µm for 1/16" OD + Holder, stainless steel (600 bar) | - | |
| XRPRCS35 | Replacement Precolumn Filter 0.5 µm for 1/16" OD, stainless steel (600 bar) | - |
- 2-3 days
- 1-2 weeks
- 3-4 weeks
