YMC-Pack Diol SEC

YMC-Pack Diol SEC

YMC-Pack Diol SEC

    UHPLC/HPLC columns for SEC

    YMC-Pack Diol is available in four tightly controlled porosities and is, therefore, suitable for separation and molecular weight determination of a wide range of peptides, proteins, oligonucleotides, carbohydrates and other biopolymers with molecular weights of approx 200 to several hundred thousand.
    Furthermore, YMC offers 3 different particle sizes with a wide range of column dimensions suitable for UHPLC/HPLC to preparative scale separations.

    The silica is derivatised with 1,2-dihydroxypropane and, with the variety of pore size available, allows analyses over a broad range of peptide to large proteins.

    YMC-Pack Diol columns are characterised by:

    • excellent reproducibility
    • long-term stability
    • improved resolution with 2 µm
    • easy method transfer from HPLC to UHPLC
    • cost-efficiency
    large pore size = large MW's
    small pore size = small MW's
    Sugar analysis

    Principles of separation

    Size Exclusion Chromatography (SEC), also referred to as Gel Filtration Chromatography (GFC) or Gel Permeation Chromatography (GPC), is a mode of liquid chromatography in which the components of a mixture are separated according to differences in their size. It is an important tool for the analysis and separation of natural and synthetic biopolymers.
    Molecules with shapes such as rigid rods, random chains and spheres but with the same molecular weight behave differently in SEC. The principle of separation is based on differences in the hydrodynamic radius of the molecules in solution.
    Molecules with a larger radius elute earlier and those with the smallest radius are retained longer. The separation limit is such that only those compounds which differ by more than 10% in MW can be separated by SEC.

    Large molecules exit the column more rapidly as they cannot permeate the porous structure of stationary phase. Smaller ones with the lowest hydrodynamic volume elute with longer retention times because they are able to penetrate some or all of the pores of the stationary phase. Molecules of intermediate size elute in an intermediate position.

    Specifications

    YMC-Pack Diol
    YMC-Pack Diol-60
    YMC-Pack Diol-120
    YMC-Pack Diol-200
    YMC-Pack Diol-300
    Suitable for
    carbohydrates and peptides
    intermediate proteins
    large proteins
    very large proteins/
    monoclonal antibodies
    Recommended MW range
    <10,000 Da
    1,000 –
    100,000 Da
    5,000 –
    300,000 Da
    20,000 –
    1,000,000 Da
    Pore size 6 nm / 60 Å 12 nm / 120 Å 20 nm / 200 Å 30 nm / 300 Å
    Particle size 3, 5 µm 3, 5 µm 2, 3, 5 µm 2, 3, 5 µm
    Surface area 650 m²/g 330 m²/g 175 m²/g 100 m²/g
    Recommended pH Range 5.0 - 7.5 5.0-7.5 5.0 - 7.5 5.0 - 7.5

     

    YMC-Pack Diol stationary phases are surface modified for a versatile alternative to underivatised silica. Advantages include a shorter equilibration time as well as good selectivity. Due to this short equilibration time, the Diol phase is a good choice for SEC in comparison with underivatised silica.

    Title Document Type Language Phase Type Download
    YMC-Pack Diol SEC (HPLC/UHPLC) Care and Use Care and Use Instructions E
    Chapter Main Catalogue - YMC Biochromatography Catalogue E
    UHPLC Size Exclusion Columns YMC-Pack Diol Flyer E
    MSDS Silica based NP/RP Bulk MSDS E
    Analytical Stationary Phases Overview E
    Investigation of Pore Size, Particle Size, and Column Geometry Effects on SEC Analysis of Monoclonal Antibodies Poster E Please login
    Use of Small Particle YMC SEC and IEX Materials for Improved Characterization of Monoclonal Antibodies Poster E Please login

    Please login to see the prices (if needed insert link to login page).

    Product Description Price (EUR) Stock
    DL06S03-1546WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 6 nm, S-3 µm, 150 x 4.6 mm -
    DL06S03-2546WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 6 nm, S-3 µm, 250 x 4.6 mm -
    DL06S03-3006WT YMC-Pack Diol, SEC Column 6.0 mm i.d., 6 nm, S-3 µm, 300 x 6.0 mm -
    DL06S03-3008WT YMC-Pack Diol, SEC Column 8.0 mm i.d., 6 nm, S-3 µm, 300 x 8.0 mm -
    DL06S03-3046WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 6 nm, S-3 µm, 300 x 4.6 mm -
    DL06S05-0110CC YMC-Pack Diol, semi-preparative Guard Cartridges (10 mm i.d.), 12 nm, S-5 µm, 10 x 10.0 mm -
    DL06S05-2506WT YMC-Pack Diol, SEC Column 6.0 mm i.d., 6 nm, S-5 µm, 250 x 6.0 mm -
    DL06S05-2510WT YMC-Pack Diol, semi-preparative SEC column (10.0 mm i.d.), 6 nm, S-5 µm, 250 x 10.0 mm -
    DL06S05-2520WT YMC-Pack Diol, semi-preparative SEC column (20.0 mm i.d.), 6 nm, S-5 µm, 250 x 20.0 mm -
    DL06S05-2530WT YMC-Pack Diol, semi-preparative SEC column (30.0 mm i.d.), 6 nm, S-5 µm, 250 x 30.0 mm -
    DL06S05-2546WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 6 nm, S-5 µm, 250 x 4.6 mm -
    DL06S05-3006WT YMC-Pack Diol, SEC Column 6.0 mm i.d., 6 nm, S-5 µm, 300 x 6.0 mm -
    DL06S05-3008WT YMC-Pack Diol, SEC Column 8.0 mm i.d., 6 nm, S-5 µm, 300 x 8.0 mm -
    DL06S05-3010WT YMC-Pack Diol, semi-preparative SEC column (10.0 mm i.d.), 6 nm, S-5 µm, 300 x 10.0 mm -
    DL06S05-3046WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 6 nm, S-5 µm, 300 x 4.6 mm -
    DL06S05-5006WT YMC-Pack Diol, SEC Column 6.0 mm i.d., 6 nm, S-5 µm, 500 x 6.0 mm -
    DL06S05-5008WT YMC-Pack Diol, SEC Column 8.0 mm i.d., 6 nm, S-5 µm, 500 x 8.0 mm -
    DL06S05-5010WT YMC-Pack Diol, semi-preparative SEC column (10.0 mm i.d.), 6 nm, S-5 µm, 500 x 10.0 mm -
    DL06S05-5020WT YMC-Pack Diol, semi-preparative SEC column (20.0 mm i.d.), 6 nm, S-5 µm, 500 x 20.0 mm -
    DL12S03-0104GC YMC-Pack Diol, Analytical Guard Cartridges (4.0 mm i.d.), 12 nm, S-3µm, 10 x 4.0 mm -
    DL12S03-1546WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 12 nm, S-3 µm, 150 x 4.6 mm -
    DL12S03-2546WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 12 nm, S-3 µm, 250 x 4.6 mm -
    DL12S03-3006WT YMC-Pack Diol, SEC Column 6.0 mm i.d., 12 nm, S-3 µm, 300 x 6.0 mm -
    DL12S03-3008WT YMC-Pack Diol, SEC Column 8.0 mm i.d., 12 nm, S-3 µm, 300 x 8.0 mm -
    DL12S03-3046WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 12 nm, S-3 µm, 300 x 4.6 mm -
    DL12S05-0104GC YMC-Pack Diol, Analytical Guard Cartridges (4.0 mm i.d.), 12 nm, S-5µm, 10 x 4.0 mm -
    DL12S05-0110CC YMC-Pack Diol, semi-preparative Guard Cartridges (10.0 mm i.d.), 12 nm, S-5µm, 10 x 10,0 mm -
    DL12S05-0120CC YMC-Pack Diol, semi-preparative Guard Cartridges (20.0 mm i.d.), 12 nm, S-5µm, 10 x 20,0 mm -
    DL12S05-01Q1GC YMC-Pack Diol, Analytical Guard Cartridges (2.1 mm i.d.)12 nm, S-5µm, 10 x 2.1 mm -
    DL12S05-0308WTG YMC-Pack Diol, SEC Guard Column (8.0 mm i.d.), 12 nm, S-5 µm, 30 x 8.0 mm -
    DL12S05-0310WTG YMC-Pack Diol, semi-preparative Guard column (8.0 mm i.d.), 12 nm, S-5 µm, 30 x 10.0 mm -
    DL12S05-10Q1QT YMC-Pack Diol, Analytical SEC column (2.1 mm i.d.), 12 nm, S-5µm, 100 x 2.1 mm -
    DL12S05-1546WT YMC-Pack Diol, Analytical SEC Column (4.6 mm i.d.), 12 nm, S-5µm, 150 x 4.6 mm -
    DL12S05-2504QT YMC-Pack Diol, Analytical SEC column (4.0 mm i.d.), 12 nm, S-5µm, 250 x 4.0 mm -
    DL12S05-2506WT YMC-Pack Diol, SEC Column 6.0 mm i.d., 12 nm, S-5 µm, 250 x 6.0 mm -
    DL12S05-2510WT YMC-Pack Diol, semi-preparative SEC column (10.0 mm i.d.), 12 nm, S-5 µm, 250 x 10.0 mm -
    DL12S05-2520WT YMC-Pack Diol, semi-preparative SEC column (20.0 mm i.d.), 12 nm, S-5 µm, 250 x 20.0 mm -
    DL12S05-2530WT YMC-Pack Diol, semi-preparative SEC column (30.0 mm i.d.), 12 nm, S-5 µm, 250 x 30.0 mm -
    DL12S05-2546WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 12 nm, S-5 µm, 250 x 4.6 mm -
    DL12S05-3006WT YMC-Pack Diol, SEC Column 6.0 mm i.d., 12 nm, S-5 µm, 300 x 6.0 mm -
    DL12S05-3008WT YMC-Pack Diol, SEC Column 8.0 mm i.d., 12 nm, S-5 µm, 300 x 8.0 mm -
    DL12S05-3010WT YMC-Pack Diol, semi-preparative SEC column (10.0 mm i.d.), 12 nm, S-5 µm, 300 x 10.0 mm -
    DL12S05-3020WT YMC-Pack Diol, semi-preparative SEC column (20.0 mm i.d.), 12 nm, S-5 µm, 300 x 20.0 mm -
    DL12S05-3046WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 12 nm, S-5 µm, 300 x 4.6 mm -
    DL12S05-5006WT YMC-Pack Diol, SEC Column 6.0 mm i.d., 12 nm, S-5 µm, 500 x 6.0 mm -
    DL12S05-5008WT YMC-Pack Diol, SEC Column 8.0 mm i.d., 12 nm, S-5 µm, 500 x 8.0 mm -
    DL12S05-5010WT YMC-Pack Diol, semi-preparative SEC column (10.0 mm i.d.), 12 nm, S-5 µm, 500 x 10.0 mm -
    DL20S02-1546PTH YMC-Pack Diol UHPLC, SEC Column 4.6 mm i.d., 20 nm, S-2 µm, 150 x 4.6 mm -
    DL20S02-3046PTH YMC-Pack Diol UHPLC, SEC Column 4.6 mm i.d., 20 nm, S-2 µm, 300 x 4.6 mm -
    DL20S03-0104GC YMC-Pack Diol, Analytical Guard Cartridges (4.0 mm i.d.), 20 nm, S-3µm, 10 x 4.0 mm -
    DL20S03-1508WT YMC-Pack Diol, SEC Column 8.0 mm i.d., 20 nm, S-3 µm, 150 x 8.0 mm -
    DL20S03-1546WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 20 nm, S-3 µm, 150 x 4.6 mm -
    DL20S03-2546WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 20 nm, S-3 µm, 250 x 4.6 mm -
    DL20S03-3006WT YMC-Pack Diol, SEC Column 6.0 mm i.d., 20 nm, S-3 µm, 300 x 6.0 mm -
    DL20S03-3008WT YMC-Pack Diol, SEC Column 8.0 mm i.d., 20 nm, S-3 µm, 300 x 8.0 mm -
    DL20S03-3046WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 20 nm, S-3 µm, 300 x 4.6 mm -
    DL20S05-0104GC YMC-Pack Diol, Analytical Guard Cartridges (4.0 mm i.d.), 20 nm, S-5µm, 10 x 4.0 mm -
    DL20S05-0110CC YMC-Pack Diol, semi-preparative Guard Cartridges (10.0 mm i.d.), 20 nm, S-5µm, 10 x 10,0 mm -
    DL20S05-0120CC YMC-Pack Diol, semi-preparative Guard Cartridges (20.0 mm i.d.), 20 nm, S-5µm, 10 x 20,0 mm -
    DL20S05-0308WTG YMC-Pack Diol, SEC Guard Column (8.0 mm i.d.), 20 nm, S-5 µm, 30 x 8.0 mm -
    DL20S05-1508WT YMC-Pack Diol, SEC Column 8.0 mm i.d., 20 nm, S-5 µm, 150 x 8.0 mm -
    DL20S05-2506WT YMC-Pack Diol, SEC Column 6.0 mm i.d., 20 nm, S-5 µm, 250 x 6.0 mm -
    DL20S05-2510WT YMC-Pack Diol, semi-preparative SEC column (10.0 mm i.d.), 20 nm, S-5 µm, 250 x 10.0 mm -
    DL20S05-2520WT YMC-Pack Diol, semi-preparative SEC column (20.0 mm i.d.), 20 nm, S-5 µm, 250 x 20.0 mm -
    DL20S05-2530WT YMC-Pack Diol, semi-preparative SEC column (30.0 mm i.d.), 20 nm, S-5 µm, 250 x 30.0 mm -
    DL20S05-2546WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 20 nm, S-5 µm, 250 x 4.6 mm -
    DL20S05-3006WT YMC-Pack Diol, SEC Column 6.0 mm i.d., 20 nm, S-5 µm, 300 x 6.0 mm -
    DL20S05-3008WT YMC-Pack Diol, SEC Column 8.0 mm i.d., 20 nm, S-5 µm, 300 x 8.0 mm -
    DL20S05-3010WT YMC-Pack Diol, semi-preparative SEC column (10.0 mm i.d.), 20 nm, S-5 µm, 300 x 10.0 mm -
    DL20S05-3046WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 20 nm, S-5 µm, 300 x 4.6 mm -
    DL20S05-5006WT YMC-Pack Diol, SEC Column 6.0 mm i.d., 20 nm, S-5 µm, 500 x 6.0 mm -
    DL20S05-5008WT YMC-Pack Diol, SEC Column 8.0 mm i.d., 20 nm, S-5 µm, 500 x 8.0 mm -
    DL20S05-5010WT YMC-Pack Diol, semi-preparative SEC column (10.0 mm i.d.), 20 nm, S-5 µm, 500 x 10.0 mm -
    DL30S02-1546PTH YMC-Pack Diol UHPLC, SEC Column 4.6 mm i.d., 30 nm, S-2 µm, 150 x 4.6 mm -
    DL30S02-3046PTH YMC-Pack Diol UHPLC, SEC Column 4.6 mm i.d., 30 nm, S-2 µm, 300 x 4.6 mm -
    DL30S03-0104GC YMC-Pack Diol, Analytical Guard Cartridges (4.0 mm i.d.), 30 nm, S-3µm, 10 x 4.0 mm -
    DL30S03-1508WT YMC-Pack Diol, SEC Column 8.0 mm i.d., 30 nm, S-3 µm, 150 x 8.0 mm -
    DL30S03-1546WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 30 nm, S-3 µm, 150 x 4.6 mm -
    DL30S03-2546WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 30 nm, S-3 µm, 250 x 4.6 mm -
    DL30S03-3006WT YMC-Pack Diol, SEC Column 6.0 mm i.d., 30 nm, S-3 µm, 300 x 6.0 mm -
    DL30S03-3008WT YMC-Pack Diol, SEC Column 8.0 mm i.d., 30 nm, S-3 µm, 300 x 8.0 mm -
    DL30S03-3046WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 30 nm, S-3 µm, 300 x 4.6 mm -
    DL30S05-0104GC YMC-Pack Diol, Analytical Guard Cartridges (4.0 mm i.d.), 30 nm, S-5µm, 10 x 4.0 mm -
    DL30S05-0105WFG YMC-Pack Diol, SEC Guard columns (5.0 mm i.d.), 30 nm, S-5 µm, 10 x 5.0 mm -
    DL30S05-0110CC YMC-Pack Diol, semi-preparative Guard Cartridges (10.0 mm i.d.),30 nm, S-5µm, 10 x 10,0 mm -
    DL30S05-0120CC YMC-Pack Diol, semi-preparative Guard Cartridges (20.0 mm i.d.), 30 nm, S-5µm, 10 x 20,0 mm -
    DL30S05-0204GC YMC-Pack Diol, Analytical Guard Cartridges (4.0 mm i.d.), 30 nm, S-5µm, 20 x 4.0 mm -
    DL30S05-0308WTG YMC-Pack Diol, SEC Guard column (8.0 mm i.d.), 30 nm, S-5 µm, 30 x 8.0 mm -
    DL30S05-0310WTG YMC-Pack Diol, semi-preparative Guard column (10.0 mm i.d.), 30 nm, S-5 µm, 30 x 10.0 mm -
    DL30S05-1503QT YMC-Pack Diol, Analytical SEC column (3.0 mm i.d.), 30 nm, S-5µm, 150 x 3.0 mm -
    DL30S05-1508WT YMC-Pack Diol, SEC Column 8.0 mm i.d., 30 nm, S-3 µm, 150 x 8.0 mm -
    DL30S05-2506WT YMC-Pack Diol, SEC Column 6.0 mm i.d., 30 nm, S-5 µm, 250 x 6.0 mm -
    DL30S05-2510WT YMC-Pack Diol, semi-preparative SEC column (10.0 mm i.d.), 30 nm, S-5 µm, 250 x 10.0 mm -
    DL30S05-2520WT YMC-Pack Diol, semi-preparative SEC column (20.0 mm i.d.), 30 nm, S-5 µm, 250 x 20.0 mm -
    DL30S05-2530WT YMC-Pack Diol, semi-preparative SEC column (30.0 mm i.d.), 30 nm, S-5 µm, 250 x 30.0 mm -
    DL30S05-2546WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 30 nm, S-5 µm, 250 x 4.6 mm -
    DL30S05-3006WT YMC-Pack Diol, SEC Column 6.0 mm i.d., 30 nm, S-5 µm, 300 x 6.0 mm -
    DL30S05-3008WT YMC-Pack Diol, SEC Column 8.0 mm i.d., 30 nm, S-5 µm, 300 x 8.0 mm -
    DL30S05-3010WT YMC-Pack Diol, semi-preparative SEC column (10.0 mm i.d.), 30 nm, S-5 µm, 300 x 10.0 mm -
    DL30S05-3020WT YMC-Pack Diol, semi-preparative SEC column (20.0 mm i.d.), 30 nm, S-5 µm, 300 x 20.0 mm -
    DL30S05-3046WT YMC-Pack Diol, SEC Column 4.6 mm i.d., 30 nm, S-5 µm, 300 x 4.6 mm -
    DL30S05-5006WT YMC-Pack Diol, SEC Column 6.0 mm i.d., 30 nm, S-5 µm, 500 x 6.0 mm -
    DL30S05-5008WT YMC-Pack Diol, SEC Column 8.0 mm i.d., 30 nm, S-5 µm, 500 x 8.0 mm -
    DL30S05-5010WT YMC-Pack Diol, semi-preparative SEC column (10.0 mm i.d.), 30 nm, S-5 µm, 500 x 10.0 mm -
    DLG12S11-2510WT YMC-Pack Diol-HG, semi-preparative SEC column (10.0 mm i.d.), 12 nm, S-10 µm, 250 x 10.0 mm -
    DLG12S11-2520WT YMC-Pack Diol-HG, semi-preparative SEC column (20.0 mm i.d.), 12 nm, S-10 µm, 250 x 20.0 mm -
    DLG12S11-3020WT YMC-Pack Diol-HG, semi-preparative SEC column (20.0 mm i.d.), 12 nm, S-10 µm, 300 x 20.0 mm -
    DLG12S11-5020WT YMC-Pack Diol-HG, semi-preparative SEC column (20.0 mm i.d.), 12 nm, S-10 µm, 500 x 20.0 mm -
    • 2-3 days
    • 1-2 weeks
    • 3-4 weeks

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    Contact

    • Dr. Daniel Eßer
    • Product Manager
    • Analytical Chromatography
    • ph. +49 (0)2064 427-280