A recently published protocol paper summarises how to set up robust HIC methods. The paper focuses on the analysis of intact proteins and mAbs. Prof. Eeltink’s group (University of Brussels/VUB) present helpful and detailed aspects especially for:

• How to set-up and maintain the system to achieve robust performance
• The effect of sample-preparation conditions on sample breakthroughs
• Run-to-run repeatability for 5 different columns obtained from 3 different column lots

The samples analysed are a mixture of intact proteins or monoclonal antibodies. The column of choice is YMC’s BioPro HIC HT column.

The full paper is freely accessible! You want know more about the applied BioPro HIC HT columns? Download the YMC BioLC catalogue below.

siRNAs are a powerful tool for genetic analysis and treatment of metabolic diseases. siRNAs can be analysed as duplex by HPLC under non-denaturing conditions to control duplex formation and the amount of excess single strands. In addition, non-hybridized single stranded RNA can be studied under denaturing conditions. In both cases, IP-RP and AEX are the most commonly used methods.

In this Application Note the analysis of a siRNA duplex under denaturing and non-denaturing conditions is shown. A bioinert YMC-Accura Triart Bio C18 UHPLC column is used in IP-RP mode, while a BioPro IEX QF column is used in AEX mode.

To obtain a full copy of this Application Note, please see below.

For more information on YMC-Accura Triart and BioPro IEX columns check out the YMC-BioLC catalogue below.