YMC-BioPro IEX

YMC-BioPro IEX

YMC-BioPro IEX

    HPLC columns for high resolution IEX applications

    Ion exchange chromatography (IEX) is widely used for analysis and purification of biomolecules. YMC-BioPro ion exchange columns are specifically designed for separation of proteins, peptides and nucleic acids.
    YMC-BioPro IEX columns are based on porous and non-porous hydrophilic polymer beads with low nonspecific adsorption. They also show higher binding capacity and higher recovery of biomolecules compared to conventional IEX columns. The completely spherical, monodisperse beads, together with optimal packing technology, provide high theoretical plate numbers and symmetrical peak shapes!

    Application Columns Compound name
    DNA fragments YMC-BioPro QA-F DNA fragments
    Lambda DNA Hind Ⅲ/EcoRⅠ restriction fragments YMC-BioPro QA-F Lambda DNA HindⅢ/EcoRⅠdigest (130-23,130 bp)
    Monoclonal antibody (MAb) YMC-BioPro SP-F Humanized monoclonal IgG1
    Peptide mapping YMC-BioPro QA Peptide mapping
    Plasmid pBR322 and pBR322 Hae III restriction fragments YMC-BioPro QA-F Plasmid pBR322 Hae III digest (8-587 bp) Plasmid pBR322 (4361 bp)
    Proteins in human serum YMC-BioPro QA IgG Transferrin Albumin
    Proteins YMC-BioPro QA Ovalbumin Trypsin inhibitor (soybean)
    Specifications
      YMC-BioPro QA YMC-BioPro SP YMC-BioPro QA-F YMC-BioPro SP-F
    Matrix Hydrophilic polymer bead
    Particle Size [µm] 5 3, 5
    Pore Size [nm / Å] 100 / 1000 Non-porous
    Charged group -CH2N+(CH3)3 -(CH2)3SO3- -CH2N+(CH3)3 -(CH2)3SO3-
    Counter ion Cl- Na+ Cl- Na+
    Ion-exchange capacity [meq/mL resin] 0.075-0.100 0.070-0.095 0.075-0.110 0.230-0.290
    Dynamic binding capacity [mg/mL resin] 110-150 (BSA) 70-100 (human IgG) 12-20 (BSA) 10-18 (human IgG)
    pH range 2.0 - 12.0

     

    YMC-BioPro QA/SP
      YMC-BioPro QA YMC-BioPro SP
    Matrix Hydrophilic polymer bead
    Particle Size 5 µm
    Pore Size 100 nm / 1000 Å
    Charged group -CH2N+(CH3)3 -(CH2)3SO3-
    Counter ion Cl- Na+
    Ion-exchange capacity 0.075-0.100 meq/ml-resin 0.070-0.095 meq/ml-resin
    Dynamic binding capacity 110-150 mg BSA/ml-resin 70-100 mg human IgG/ml-resin
    pH range 2.0 - 12.0

     

    The porous type of YMC-BioPro shows great absorption capacity and excellent recovery, and is especially suitable for semi-preparative separations of proteins and antibodies.

    Compared with conventional porous polymer anion-exchange columns, YMC-BioPro phases give higher dynamic binding capacities (DBC) and recovery rates. This indicates that YMC-BioPro has a much lower nonspecific adsorption compared to conventional columns.

     

    YMC-BioPro QA-F/SP-F
      YMC-BioPro QA-F YMC-BioPro SP-F
    Matrix Hydrophilic polymer bead
    Particle Size 3, 5 µm
    Pore Size Non-porous
    Charged group -CH2N+(CH3)3 -(CH2)3SO3-
    Counter ion Cl- Na+
    Ion-exchange capacity 0.075-0.110 meq/ml-resin 0.230-0.290 meq/ml-resin
    Dynamic binding capacity 12-20 mg BSA/ml-resin 10-18 mg human IgG/ml-resin
    pH range 2.0 - 12.0

     

    The non-porous YMC-BioPro particles offer high efficiency, exceptional resolution, and low operating pressures.

    Title Document Type Language Phase Type Download
    YMC Phases for Biochromatography Brochure E
    YMC-BioPro Columns Care and Use Care and Use Instructions E
    Analytical Stationary Phases Overview E
    An Evaluation of YMC’s Strong Cation-Exchange Stationary Phase for the Analysis of Charged Isoforms of a Commercially Available Antibody Drug Poster E Please login
    Use of Small Particle YMC SEC and IEX Materials for Improved Characterization of Monoclonal Antibodies Poster E Please login

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    Product Description Price (EUR) Stock
    QAA0S05-0346WP YMC-BioPro QA, (4.6 mm i.d.), 100 nm, 5 µm, 30 x 4.6 mm -
    QAA0S05-0546WP YMC-BioPro QA, (4.6 mm i.d.), 100 nm, 5 µm, 50 x 4.6 mm -
    QAA0S05-1046WP YMC-BioPro QA, (4.6 mm i.d.), 100 nm, 5 µm, 100 x 4.6 mm -
    QF00S03-0346WP YMC BioPro QA-F, non-porous*, (4.6 mm i.d.), 3 µm, 30 x 4.6 mm -
    QF00S03-0546WP YMC BioPro QA-F, non-porous*, (4.6 mm i.d.), 3 µm, 50 x 4.6 mm -
    QF00S03-1046WP YMC BioPro QA-F, non-porous*, (4.6 mm i.d.), 3 µm, 100 x 4.6 mm -
    QF00S05-0346WP YMC-BioPro QA-F, non-porous*, (4.6 mm i.d.), 5 µm, 30 x 4.6 mm -
    QF00S05-0546WP YMC-BioPro QA-F, non-porous*, (4.6 mm i.d.), 5 µm, 50 x 4.6 mm -
    QF00S05-1046WP YMC-BioPro QA-F, non-porous*, (4.6 mm i.d.), 5 µm, 100 x 4.6 mm -
    QF00S05-1546WP YMC-BioPro QA-F, non-porous, S-5 µm, 150 x 4.6 mm -
    SF00S03-0346WP YMC-BioPro SP-F, non-porous, S-3 µm, 30 x 4.6 mm -
    SF00S03-0546WP YMC-BioPro SP-F, non-porous, S-3 µm, 50 x 4.6 mm -
    SF00S03-1046WP YMC-BioPro SP-F, non-porous, S-3 µm, 100 x 4.6 mm -
    SF00S05-0346WP YMC-BioPro SP-F, non-porous, S-5 µm, 30 x 4.6 mm -
    SF00S05-0546WP YMC-BioPro SP-F, non-porous, S-5 µm, 50 x 4.6 mm -
    SF00S05-1046WP YMC-BioPro SP-F, non-porous, S-5 µm, 100 x 4.6 mm -
    SF00S05-1546WP YMC-BioPro SP-F, non-porous, S-5 µm, 150 x 4.6 mm -
    SPA0S05-0346WP YMC-BioPro SP, (4.6 mm i.d.), 100 nm, 5 µm, 30 x 4.6 mm -
    SPA0S05-0546WP YMC-BioPro SP, (4.6 mm i.d.), 100 nm, 5 µm, 50 x 4.6 mm -
    SPA0S05-1046WP YMC-BioPro SP, (4.6 mm i.d.), 100 nm, 5 µm, 100 x 4.6 mm -
    • 2-3 days
    • 1-2 weeks
    • 3-4 weeks

    Related Categories

    Contact

    • Dr. Daniel Eßer
    • Product Manager
    • Analytical Chromatography
    • ph. +49 (0)2064 427-280